Journal: PLoS ONE

Article Title: An In Vitro Cord Formation Assay Identifies Unique Vascular Phenotypes Associated with Angiogenic Growth Factors

doi: 10.1371/journal.pone.0106901

Figure Lengend Snippet: ( A ) Basal cords made with ADSCs and ECFCs without the addition of growth factors were treated with IgG, or anti-VEGF or anti-HGF antibodies and stained for cords (CD31; green), smooth muscle actin (SMA; red), and nuclei (Hoechst 33342; blue). ( B ) Basal cords treated with IgG, anti-VEGF, or anti-HGF were quantified on the ArrayScan and the total tube area (left) and SMA index (right) are shown. * p<0.0001 vs basal. n = 3 per group. Scale bars are 250 µm.

Article Snippet: Cord formation images were capture with either a Cellomics Arrayscan VTI or an Acumen eX3 microplate cytometer.

Techniques: Staining

Journal: PLoS ONE

Article Title: An In Vitro Cord Formation Assay Identifies Unique Vascular Phenotypes Associated with Angiogenic Growth Factors

doi: 10.1371/journal.pone.0106901

Figure Lengend Snippet: ( A ) ADSC and ECFC co-cultures were stimulated with angiogenic growth factors; VEGF, HGF, bFGF, or EGF and stained for cords (CD31; green), smooth muscle actin (SMA; red), and nuclei (Hoechst 33342; blue). ( B ) To assess whether the cord induction was dependent on VEGF, cords were treated with the different angiogenic factors plus an anti-VEGF antibody (Bevacizumab; Bev). ( C ) ArrayScan quantification of total tube area, branching index, length to width ratio and SMA index are shown as an indication of phenotypes associated with the VEGF, HGF, bFGF, or EGF driven cords. n = 3 per group. ‡ = p<0.05 vs. BM. † = p<0.05 vs. VEGF. # = p<0.05 vs. bFGF. + = p<0.05 vs. HGF. * = p<0.05 vs. EGF. Scale bars are 250 µm.

Article Snippet: Cord formation images were capture with either a Cellomics Arrayscan VTI or an Acumen eX3 microplate cytometer.

Techniques: Staining

Journal: PLoS ONE

Article Title: An In Vitro Cord Formation Assay Identifies Unique Vascular Phenotypes Associated with Angiogenic Growth Factors

doi: 10.1371/journal.pone.0106901

Figure Lengend Snippet: ( A ) ADSC and ECFC co-cultures were treated with combinations of angiogenic growth factors (V = VEGF, H = HGF, F = bFGF, E = EGF) with or without anti-VEGF (Bevacizumab; Bev) and stained for cords (CD31; green), smooth muscle actin (SMA; red), and nuclei (Hoechst 33342; blue). ( B ) ArrayScan quantifications of total tube area, branching index, length to width ratio, and SMA index of samples treated with combinations of growth factors with and without bevacizumab. n = 3 per group. * = p<0.05 vs. V+H. † = p<0.05 vs. V+F. ‡ = p<0.05 vs. H+E. # = p<0.05 vs. F+E. + = p<0.05 vs. H+E+F. Scale bars are 250 µm.

Article Snippet: Cord formation images were capture with either a Cellomics Arrayscan VTI or an Acumen eX3 microplate cytometer.

Techniques: Staining

Journal: PLoS ONE

Article Title: An In Vitro Cord Formation Assay Identifies Unique Vascular Phenotypes Associated with Angiogenic Growth Factors

doi: 10.1371/journal.pone.0106901

Figure Lengend Snippet: Basal, VEGF-, bFGF-, or EGF-driven cords were treated with a VEGFR-2 antibody (IMC-1121B; Ramucirumab; Ram), a bFGF antibody (anti-bFGF), or an EGFR inhibitor (Gefitinib) and the percent inhibition of total tube area from the ArrayScan was graphed. n = 3 per group.

Article Snippet: Cord formation images were capture with either a Cellomics Arrayscan VTI or an Acumen eX3 microplate cytometer.

Techniques: Inhibition

Journal: PLoS ONE

Article Title: An In Vitro Cord Formation Assay Identifies Unique Vascular Phenotypes Associated with Angiogenic Growth Factors

doi: 10.1371/journal.pone.0106901

Figure Lengend Snippet: ( A ) Co-cultures of ADSCs and ECFCs were treated with a TGF-β inhibitor (LY2157299) and stained for cords (CD31; green), smooth muscle actin (SMA; red), and nuclei (Hoechst 33342; blue). ( B ) ArrayScan quantifications of total tube area following treatment with LY2157299 in basal medium (left) versus VEGF driven cords (right). n = 6 per group. ( C ) Mice harboring A549 tumor xenografts were treated with a TGF-β inhibitor (LY2157299), an inactive control (LY596144), or sunitinib (Sutent; SU11248) for 5 days. Tumors were fixed, sectioned, stained for tumor vessels (CD31; green), and quantified using Image J. * = p<0.05 vs. control. n = 4–6 per treatment group. Scale bars in A are 250 µm. Scale bars in B are 50 µm.

Article Snippet: Cord formation images were capture with either a Cellomics Arrayscan VTI or an Acumen eX3 microplate cytometer.

Techniques: Staining